1. Dna Slot Blot Protocol Assay

The DNA (ng range) to stain is most probably denaturalized ssDNA (Urea/SDS backextraction from the TRIZOL inter-phase) and it's blotted on a positively charged Nylon membrane. A dsDNA antibody. Dot-blot is one of biological methods that are normally used in research laboratories and especially in the diagnostics. It is the most commonly used method for identification and immunodetection of particular proteins which may be markers of various diseases. The nucleic acid mixture is blotted to a membrane where the hybridisation is carried out. The difference between dot and slot blot procedures is in the way that the nucleic acid mixture is blotted onto the membrane. In dot blotting the nucleic acids are blotted as circular blots, whereas in slot blotting they are blotted into rectangular slots.

Dna Slot Blot Protocol Assay

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